FREE LC MS METHOD VALIDATION : INTERNATIONAL COURSE

This course is offered as a MOOC during Nov 28, 2017 – Feb 09, 2018

This course – LC-MS Method Validation – is a practice-oriented on-line course on validation of analytical methods, specifically using LC-MS as technique. The course introduces the main concepts and mathematical apparatus of validation, covers the most important method performance parameters and ways of estimating them. The course is largely based on the recently published two-part tutorial review:

Tutorial review on validation of liquid chromatography–mass spectrometry methods: Part I. A. Kruve, R. Rebane, K. Kipper, M.-L. Oldekop, H. Evard, K. Herodes, P. Ravio, I. Leito. Anal. Chim. Acta 2015, 870, 29-44 Tutorial review on validation of liquid chromatography–mass spectrometry methods: Part II. A. Kruve, R. Rebane, K. Kipper, M.-L. Oldekop, H. Evard, K. Herodes, P. Ravio, I. Leito. Anal. Chim. Acta 2015, 870, 8-28

The course contains lectures, practical exercises and numerous tests for self-testing. In spite of being introductory, the course intends to offer sufficient knowledge and skills for carrying out validation for most of the common LC-MS analyses in routine laboratory environment. The real-life analysis situations for which there are either examples or self-tests are for example pesticide analyses in fruits and vegetables, perfluoroalkyl acids in water, antibiotics in blood serum, glyphosate and AMPA in surface water, etc. It is important to stress, that for successful validation experience (both in analytical chemistry as such and also specifically in validation) is crucial and this can be acquired only through practice.

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The course contains:

Course introduction

1. Validation: General 

2. Selectivity and identity confirmation 

2.1. Selectivity: LC selectivity

Self-test 2.1

2.2. Selectivity: detector-side (MS) selectivity

Self test 2.2

2.3. Selectivity examples

2.4. Introduction to identity confirmation

Self test 2.4

2.5. Identity confirmation: retention time

2.6. Identity confirmation by MS

2.7. Identity confirmation examples

Self test 2.7

3. Linearity of signal, linear range, sensitivity 

4. Precision 

5. Trueness 

6. Precision and trueness: some additional aspects 

7. Accuracy 

8. Stability 

9. LoD and LoQ 

10. Ruggedness, robustness 

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